Antimicrobial Activity Test of Medicinal Plant Extract Using Antimicrobial Disc and Filter Paper

Plant extracts contain secondary metabolites such as flavonoids and phenolic compounds with antibiotic activity. Antibiotic activity test can be done by using the Kirby-Bauer disc method which is indicated formation of a clear zone. This research was conducted Moringa oleifera leaves Opuntia cochenillifera cladode extracted ethyl acetate solvent. against Staphylococcus aureus bacteria in Mannitol Salt Agar Luria Bertani media. The discs used are Antimicrobial Disc Oxoid® Filter Paper. results this study show that use Paper shows no statistical difference zone (t-value = 0,45; p-value 0,655; α 95%). While Clear Zone on gave better than were statistically significant 2,46; 0,02; These findings indicate filter paper an inexpensive alternative for significantly different result compared to commercial disc. However, (MSA) (LBA).